Ro5-4864 1 MG Blotters

Ro5 4864 1 MG Blotters

Ro5 4864 blotters contain 1 milligram of active substance (Ro5-4864).

Ro5-4864 is a substance that is only sold for research purposes and is therefore not intended for consumption.

Ro5-4864 remains stable for up to 2 years when stored in the correct environment. Ro5-4864 is intended for research purposes and not for human consumption. Therefore, it is important to store the product out of the reach of children and pets. 1cP-LSD 150 MCG Арт-блоттеры

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Research Methodology (Materials and Methods)

• 4.1 Type of Research: Experimental, analytical, and in vitro toxicological research.

• 4.2 Study Design: Laboratory analysis of blotter samples followed by cell-based assays.

• 4.3 Materials:

  • Substances: Ro5-4864 reference standard (commercial supplier, if available). Blotter samples (obtained through forensic collaboration). Cell culture media and reagents. Fluorescent dyes: JC-1 or TMRE (for mitochondrial membrane potential, ΔΨm), Annexin V-FITC/PI kit (for apoptosis).

  • Apparatus: UPLC-MS/MS system, LC-HRMS (QTOF), cell culture hood and incubator, fluorescence microplate reader, flow cytometer (optional for Annexin V).

• 4.4 Methods:

  • Sample Preparation & Chemical Analysis:

    • Extraction: Extract individual blotters in methanol/acetonitrile with an internal standard (e.g., deuterated benzodiazepine). Sonicate.

    • Quantification (UPLC-MS/MS): Develop a method using a C18 column. Mobile phase: ammonium formate/acetonitrile. MS/MS in ESI+ mode with MRM transitions for Ro5-4864. Validate for linearity, precision, accuracy, LOQ.

    • Dosage Uniformity: Analyze 10 blotters from at least two different sheets. Calculate mean, SD, RSD%.

    • Contaminant Screening (LC-HRMS): Perform non-targeted DDA analysis. Screen for related structures (e.g., diazepam, other chloro-benzodiazepines, precursor molecules).

Ro5-4864 properties

Synonym: 4′-chlorodiazepam
CAS Number: 14439-61-3
Formula: C ₁₆ H ₁₂ Cl ₂ N ₂ O
Molar mass: 319.19 g/mol
Purity: >98%

Hazard statement

Ro5-4864 may cause respiratory irritation.
Ro5-4864 may cause eye irritation.
Discharging research chemicals into the environment is prohibited.

Ro5-4864 IF IN EYES: Rinse eyes gently with water for five minutes. Remove contact lenses, if present and easy to do. O-DSMT 50mg Pellets

Ro5-4864 IN CONTACT WITH SKIN: Wash skin with soap and water.

IF INHALED: Remove victim to fresh air and keep in a position comfortable for breathing. 2C-EF 2mg Capsules